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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1QL3 Polyclonal antibody (32175-1-AP)

    WB analysis using 32175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32175-1-AP (C1QL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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C1QL3 Polyclonal antibody

C1QL3 Polyclonal Antibody for WB, ELISA
Cat No. 32175-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C1q and tumor necrosis factor-related protein 13, C1q/TNF-related protein 13, Complement C1q-like protein 3, CTRP13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32175-1-AP targets C1QL3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1QL3 fusion protein Ag37280 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent-like 3
Calculated molecular weight27 kDa
Observed molecular weight70 kDa, 27 kDa
GenBank accession numberBC127716
Gene Symbol C1QL3
Gene ID (NCBI) 389941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5VWW1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C1Q-like (C1QL) proteins bind to the adhesion G protein coupled receptor B3 (ADGRB3) and act at synapses in a subset of circuits. Complement C1q-like 3 (C1QL3, also known as CTRP13) is one of the C1QL proteins. Some C1QL3 migrated as a monomer (~26 kDa) and some as a trimer (60-70 kDa), indicating a desired substantial yet incomplete crosslinking of monomers (PMID: 33337553). The doublet band of trimer probably represents differential posttranslational modifications (PMID: 21378161). Bands of larger molecular weights suggested either trimers cross-linked into higher molecular weight oligomers or C1QL3 cross-linked to binding partners (PMID: 33337553).

Protocols

Product Specific Protocols
WB protocol for C1QL3 antibody 32175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols