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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1QBP Recombinant antibody, PBS Only

C1QBP Recombinant Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K20

Cat no : 80490-1-PBS

Synonyms

C1QBP, gC1Q R, GC1q R protein, GC1QBP, gC1qR, Glycoprotein gC1qBP, HABP1, Hyaluronan binding protein 1, p32, p33, SF2p32

Validation Data Gallery

Applications:
  • WB analysis using 80490-1-RR (same clone as 80490-1-PBS)

    WB analysis using 80490-1-RR (same clone as 80490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80490-1-RR (C1QBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 80490-1-RR (same clone as 80490-1-PBS)

    IHC staining of human tonsillitis using 80490-1-RR (same clone as 80490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80490-1-RR (C1QBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C1QBP antibody included
  • IHC staining of human tonsillitis using 80490-1-RR (same clone as 80490-1-PBS)

    IHC staining of human tonsillitis using 80490-1-RR (same clone as 80490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80490-1-RR (C1QBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C1QBP antibody included
  • IF Staining of HeLa using 80490-1-RR (same clone as 80490-1-PBS)

    IF Staining of HeLa using 80490-1-RR (same clone as 80490-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C1QBP antibody (80490-1-RR, Clone: 3K20 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80490-1-RR (same clone as 80490-1-PBS)

    FC experiment of HeLa using 80490-1-RR (same clone as 80490-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human C1QBP (80490-1-RR, Clone:3K20) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Rabbit IgG (KFA002), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy C1QBP Ready-To-Use IHC Kit. C1QBP primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

80490-1-PBS targets C1QBP in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C1QBP fusion protein Ag19773 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent binding protein
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight32 kDa
GenBank accession numberBC013731
Gene symbol C1QBP
Gene ID (NCBI) 708
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).