Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
25514-1-AP targets C19orf70 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C19orf70 fusion protein Ag22003 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 19 open reading frame 70 |
| Calculated molecular weight | 118 aa, 13 kDa |
| Observed molecular weight | 13 kDa |
| GenBank accession number | BC042386 |
| Gene Symbol | C19orf70 |
| Gene ID (NCBI) | 125988 |
| RRID | AB_2880112 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5XKP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C19orf70, also named as QIL1 and protein P117, belongs to the UPF0433 family. This antibody is specific to C19orf70.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C19orf70 antibody 25514-1-AP | Download protocol |
| IHC protocol for C19orf70 antibody 25514-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Individual cristae within the same mitochondrion display different membrane potentials and are functionally independent. | ||
iScience Protease OMA1 modulates mitochondrial bioenergetics and ultrastructure through dynamic association with MICOS complex. | ||
Cytotherapy Stem cells from human exfoliated deciduous teeth affect mitochondria and reverse cognitive decline in a senescence-accelerated mouse prone 8 model. | ||
mBio Listeria monocytogenes Exploits Mitochondrial Contact Site and Cristae Organizing System Complex Subunit Mic10 To Promote Mitochondrial Fragmentation and Cellular Infection.
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