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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C19orf50 Polyclonal antibody (20492-1-AP)

    WB analysis using 20492-1-AP (same clone as 20492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20492-1-AP (C19orf50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20492-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C19orf50 Polyclonal antibody (20492-1-AP)

    IHC staining of mouse cerebellum using 20492-1-AP (same clone as 20492-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20492-1-AP (C19orf50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20492-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C19orf50 Polyclonal antibody (20492-1-AP)

    IHC staining of mouse cerebellum using 20492-1-AP (same clone as 20492-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20492-1-AP (C19orf50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20492-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using C19orf50 Polyclonal antibody (20492-1-AP)

    IF Staining of A549 using 20492-1-AP (same clone as 20492-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C19orf50 antibody (20492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20492-1-PBS in a different storage buffer formulation.

C19orf50 Polyclonal antibody, PBS Only

C19orf50 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20492-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20492-1-PBS targets C19orf50 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13743

Product name: Recombinant human C19orf50 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-176 aa of BC001080

Sequence: MDLPDSASRVFCGRILSMVNTDDVNAIILAQKNMLDRFEKTNEMLLNFNNLSSARLQQMSERFLHHTRTLVEMKRDLDSIFRRIRTLKGKLARQHPEAFSHIPEASFLEEEDEDPIPPSTTTTIATSEQSTGSCDTSPDTVSPSLSPGFEDLSHVQAGSPAINGRSQTDDEEMTGE

相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 50
Calculated molecular weight 176 aa, 20 kDa
Observed molecular weight28 kDa
GenBank accession numberBC001080
Gene Symbol C19orf50
Gene ID (NCBI) 79036
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQD3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C19orf50, also known as KXD1 (KxDL motif-containing protein 1), a human uncharacterized coiled-coil protein, is a BLOS1-interacting protein. C19orf50 has been shown recently to interact with BLOC-1 subunits and may play a role in lysosome movement and localization at the cell periphery. C19orf50 was evaluated in NSCLC and LUAD (PMID: 33936200, 22554196).

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