Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells |
| Positive IHC detected in | human colon tissue, human gliomas tissue, human stomach cancer tissue, mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IP | See 1 publications below |
Product Information
19420-1-AP targets C19orf43 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13747 Product name: Recombinant human C19orf43 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-176 aa of BC000216 Sequence: MAARGRRAEPQGREAPGPAGGGGGGSRWAESGSGTSPESGDEEVSGAGSSPVSGGVNLFANDGSFLELFKRKMEEEQRQRQEEPPPGPQRPDQSAAAAGPGDPKRKGGPGSTLSFVGKRRGGNKLALKTGIVAKKQKTEDEVLTSKGDAWAKYMAEVKKYKAHQCGDDDKTRPLVK 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 19 open reading frame 43 |
| Calculated molecular weight | 176 aa, 18 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC000216 |
| Gene Symbol | C19orf43 |
| Gene ID (NCBI) | 79002 |
| RRID | AB_10640586 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQ61 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C19orf43, also named as MGC2803 and fSAP18, is an Uncharacterized protein. It is 176aa,18.4kd. We used the antibody raised from the C19orf43 protein, we get a ~28kd in human placenta, 293 and A431 cell.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for C19orf43 antibody 19420-1-AP | Download protocol |
| WB protocol for C19orf43 antibody 19420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
