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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using C19orf43 Polyclonal antibody (19420-1-AP)

    WB analysis of A431 using 19420-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 19420-1-AP (C19orf43 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using C19orf43 Polyclonal antibody (19420-1-AP)

    WB analysis of HEK-293 using 19420-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19420-1-AP (C19orf43 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using C19orf43 Polyclonal antibody (19420-1-AP)

    IHC staining of human colon using 19420-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using C19orf43 Polyclonal antibody (19420-1-AP)

    IHC staining of human gliomas using 19420-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using C19orf43 Polyclonal antibody (19420-1-AP)

    IHC staining of human stomach cancer using 19420-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using C19orf43 Polyclonal antibody (19420-1-AP)

    IHC staining of rat colon using 19420-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using C19orf43 Polyclonal antibody (19420-1-AP)

    IHC staining of mouse colon using 19420-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

C19orf43 Polyclonal antibody

C19orf43 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19420-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

TRIR, TERCIR, Telomerase RNA component interacting RNase, fSAP18, Exoribonuclease TRIR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells
Positive IHC detected inhuman colon tissue, human gliomas tissue, human stomach cancer tissue, mouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19420-1-AP targets C19orf43 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C19orf43 fusion protein Ag13747 相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 43
Calculated molecular weight 176 aa, 18 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC000216
Gene Symbol C19orf43
Gene ID (NCBI) 79002
RRIDAB_10640586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQ61
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C19orf43, also named as MGC2803 and fSAP18, is an Uncharacterized protein. It is 176aa,18.4kd. We used the antibody raised from the C19orf43 protein, we get a ~28kd in human placenta, 293 and A431 cell.

Protocols

Product Specific Protocols
WB protocol for C19orf43 antibody 19420-1-APDownload protocol
IHC protocol for C19orf43 antibody 19420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Rep

TERRA R-loops connect and protect sister telomeres in mitosis

Authors - Samantha Sze
  • KD Validated
View Article