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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using C19orf36 Polyclonal antibody (25650-1-AP)

    WB analysis of mouse testis using 25650-1-AP (same clone as 25650-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25650-1-AP (C19orf36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25650-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using C19orf36 Polyclonal antibody (25650-1-AP)

    WB analysis of HepG2 using 25650-1-AP (same clone as 25650-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25650-1-AP (C19orf36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25650-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C19orf36 Polyclonal antibody (25650-1-AP)

    IHC staining of mouse testis using 25650-1-AP (same clone as 25650-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25650-1-AP (C19orf36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25650-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C19orf36 Polyclonal antibody (25650-1-AP)

    IHC staining of mouse testis using 25650-1-AP (same clone as 25650-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25650-1-AP (C19orf36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25650-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using C19orf36 Polyclonal antibody (25650-1-AP)

    IHC staining of human testis using 25650-1-AP (same clone as 25650-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25650-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using C19orf36 Polyclonal antibody (25650-1-AP)

    IHC staining of human testis using 25650-1-AP (same clone as 25650-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25650-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C19orf36 Polyclonal antibody (25650-1-AP)

    IF Staining of HepG2 using 25650-1-AP (same clone as 25650-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C19orf36 antibody (25650-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 25650-1-PBS in a different storage buffer formulation.

C19orf36 Polyclonal antibody, PBS Only

C19orf36 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25650-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Izumo sperm-egg fusion protein 4, IZUMO4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25650-1-PBS targets C19orf36 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20434

Product name: Recombinant human C19orf36 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 16-214 aa of BC014609

Sequence: HGCLHCHSNFSKKFSFYRHHVNFKSWWVGDIPVSGALLTDWSDDTMKELHLAIPAKITREKLDQVATAVYQMMDQLYQGKMYFPGYFPNELRNIFREQVHLIQNAIIESRIDCQHRCGIFQYETISCNNCTDSHVACFGYNCESSAQWKSAVQGLLNYINNWHKQDTSMSLVSPALRCLEPPHLANLTLEDAAECLKQH

相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 36
Calculated molecular weight 232 aa, 27 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC014609
Gene Symbol C19orf36
Gene ID (NCBI) 113177
RRIDAB_2880175
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ1ZYL8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IZUMO4, also named as C19orf36, is sperm 22 kDa protein c113. It has 4 isoforms with MW 17-27 kDa.

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