Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, mouse colon tissue, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 10 publications below |
| IHC | See 4 publications below |
| IF | See 4 publications below |
Product Information
11353-1-AP targets MYDGF/C19orf10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1900 Product name: Recombinant human C19orf10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-173 aa of BC010129 Sequence: MAAPSGGWNGVGASLWAALLLGAVALRPAEAVSEPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGKSYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKTAVAHRPGAFKAELSKLVIVAKASRTEL 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 19 open reading frame 10 |
| Calculated molecular weight | 173 aa, 19 kDa |
| Observed molecular weight | 15-19 kDa |
| GenBank accession number | BC010129 |
| Gene Symbol | MYDGF/C19orf10 |
| Gene ID (NCBI) | 56005 |
| RRID | AB_2207647 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969H8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYDGF/C19orf10 antibody 11353-1-AP | Download protocol |
| IHC protocol for MYDGF/C19orf10 antibody 11353-1-AP | Download protocol |
| WB protocol for MYDGF/C19orf10 antibody 11353-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice.
| ||
Nat Commun Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis. | ||
Kidney Int Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease.
| ||
Front Immunol Myeloid-derived growth factor promotes M2 macrophage polarization and attenuates Sjögren's syndrome via suppression of the CX3CL1/CX3CR1 axis | ||
Front Oncol The Clinical Relevance and Tumor Promoting Function of C19orf10 in Kidney Renal Clear Cell Carcinoma.
| ||
J Cancer C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways.
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