Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 11353-1-AP
various cells were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF/C19orf10F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11353-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 11353-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 11353-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11353-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11353-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF/C19orf10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11353-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11353-1-AP
Immunofluorescent analysis of HepG2 cells, using MYDGF/C19orf10 antibody 11353-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HepG2 using 11353-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYDGF/C19orf10 antibody (11353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

MYDGF/C19orf10 Polyclonal antibody

MYDGF/C19orf10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11353-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MYDGF, C19orf10, UPF0556 protein C19orf10, SF20, Myeloid-derived growth factor

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	DU 145 cells, HeLa cells, mouse colon tissue, Jurkat cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 9 publications below
IHC	See 4 publications below
IF	See 4 publications below

Product Information

11353-1-AP targets MYDGF/C19orf10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MYDGF/C19orf10 fusion protein Ag1900 相同性解析による交差性が予測される生物種
Full Name	chromosome 19 open reading frame 10
Calculated molecular weight	173 aa, 19 kDa
Observed molecular weight	15-19 kDa
GenBank accession number	BC010129
Gene Symbol	MYDGF/C19orf10
Gene ID (NCBI)	56005
RRID	AB_2207647
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q969H8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).

Protocols

Product Specific Protocols
WB protocol for MYDGF/C19orf10 antibody 11353-1-AP	Download protocol
IHC protocol for MYDGF/C19orf10 antibody 11353-1-AP	Download protocol
IF protocol for MYDGF/C19orf10 antibody 11353-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Sci Adv Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice. Authors - Biying Meng KO Validated View Article
human,mouse,rat	WB,IHC,IF	Kidney Int Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease. Authors - Ping Zhan KO Validated View Article
mouse	WB	Front Immunol Myeloid-derived growth factor promotes M2 macrophage polarization and attenuates Sjögren's syndrome via suppression of the CX3CL1/CX3CR1 axis Authors - Zi Yang View Article
human	WB,IHC	Front Oncol The Clinical Relevance and Tumor Promoting Function of C19orf10 in Kidney Renal Clear Cell Carcinoma. Authors - Yanxin Lu KD Validated View Article
human	IHC,WB	J Cancer C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways. Authors - Shi Li KD Validated View Article
human,mouse	WB,IHC,IF	Cell Death Dis Myeloid-derived growth factor alleviates non-alcoholic fatty liver disease alleviates in a manner involving IKKβ/NF-κB signaling Authors - Yan Ding View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11353-1-AP

WB analysis of HeLa using 11353-1-AP

WB analysis of mouse colon using 11353-1-AP

IHC staining of human liver cancer using 11353-1-AP

IHC staining of human liver cancer using 11353-1-AP

IHC staining of human liver cancer using 11353-1-AP

IHC staining of human liver cancer using 11353-1-AP

IF Staining of HepG2 using 11353-1-AP

IF Staining of HepG2 using 11353-1-AP

MYDGF/C19orf10 Polyclonal antibody

MYDGF/C19orf10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice.

Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease.

Myeloid-derived growth factor promotes M2 macrophage polarization and attenuates Sjögren's syndrome via suppression of the CX3CL1/CX3CR1 axis

The Clinical Relevance and Tumor Promoting Function of C19orf10 in Kidney Renal Clear Cell Carcinoma.

C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways.

Myeloid-derived growth factor alleviates non-alcoholic fatty liver disease alleviates in a manner involving IKKβ/NF-κB signaling