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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using C18orf21 Polyclonal antibody (24977-1-AP)

    WB analysis of COLO 320 using 24977-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24977-1-AP (C18orf21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human colon cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human pancreas using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human thyroid cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human thyroid cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human urothelial carcinoma using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human urothelial carcinoma using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human pancreas using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C18orf21 Polyclonal antibody (24977-1-AP)

    IHC staining of human colon cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using C18orf21 Polyclonal antibody (24977-1-AP)

    IF Staining of COLO 320 using 24977-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 24977-1-AP (C18orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

C18orf21 Polyclonal antibody

C18orf21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24977-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

XTP13, PNAS-131, PNAS-124, HsT3108, HBV X-transactivated gene 13 protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human colon cancer tissue, human pancreas tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24977-1-AP targets C18orf21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21782

Product name: Recombinant human C18orf21 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-220 aa of BC119766

Sequence: MRQKHYLEAAARGLHDSCPGQARYLLWAYTSSHDDKSTFEETCPYCFQLLVLDNSRVRLKPKARLTPKIQKLLNREARNYTLSFKEAKMVKKFKDSKSVLLITCKTCNRTVKHHGKSRSFVSTLKSNPATPTSKLSLKTPERRTANPNHDMSGSKGKSPASVFRTPTSGQSVSTCSSKNTSKTKKHFSQLKMLLSQNESQKIPKVDFRNFLSSLKGGLLK

相同性解析による交差性が予測される生物種
Full Name chromosome 18 open reading frame 21
Calculated molecular weight 220 aa, 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC119766
Gene Symbol C18orf21
Gene ID (NCBI) 83608
RRIDAB_2879829
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ32NC0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C18orf21, also named as XTP13, belongs to the UPF0711 family. The MW is 25-28 kDa in WB detection.

Protocols

Product Specific Protocols
IF protocol for C18orf21 antibody 24977-1-APDownload protocol
IHC protocol for C18orf21 antibody 24977-1-APDownload protocol
WB protocol for C18orf21 antibody 24977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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