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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C17orf81 Polyclonal antibody (10162-1-AP)

    WB analysis using 10162-1-AP (same clone as 10162-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10162-1-AP (C17orf81 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using C17orf81 Polyclonal antibody (10162-1-AP)

    WB analysis of HEK-293 using 10162-1-AP (same clone as 10162-1-PBS)

    WB result of C17orf81 antibody (10162-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C17orf81 transfected HEK-293 cells. This data was developed using the same antibody clone with 10162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using C17orf81 Polyclonal antibody (10162-1-AP)

    IHC staining of mouse heart using 10162-1-AP (same clone as 10162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C17orf81 Polyclonal antibody (10162-1-AP)

    IHC staining of mouse brain using 10162-1-AP (same clone as 10162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using C17orf81 Polyclonal antibody (10162-1-AP)

    IHC staining of mouse heart using 10162-1-AP (same clone as 10162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using C17orf81 Polyclonal antibody (10162-1-AP)

    IHC staining of mouse heart using 10162-1-AP (same clone as 10162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10162-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

C17orf81 Polyclonal antibody, PBS Only

C17orf81 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10162-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

MSTP071, MST071, HSPC002, ELP5, Elongator complex protein 5

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10162-1-PBS targets C17orf81 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0194

Product name: Recombinant human C17orf81 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-209 aa of BC002762

Sequence: MTPSEGARAGTGRELEMLDSLLALGGLVLLRDSVEWEGRSLLKALVKKSALCGEQVHILGCEVSEEEFREGFDSDINNRLVYHDFFRDPLNWSKTEEAFPGGPLGALRAMCKRTDPVPVTIALDSLSWLLLRLPCTTLCQVLHAVSHQDSCPGDSSSVGKVSVLGLLHEELHGPGPVGALSSLAQTEVTLGGTMGQASAHILCRRPRQR

相同性解析による交差性が予測される生物種
Full Name chromosome 17 open reading frame 81
Calculated molecular weight 35 kDa
Observed molecular weight 37 and 45 kDa
GenBank accession numberBC002762
Gene Symbol C17orf81
Gene ID (NCBI) 23587
RRIDAB_2274975
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TE02
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ELP5, also named as C17orf81, DERP6 and S-phase 2 protein, acts as subunit of the RNA polymerase II elongator complex. It's involved in TP53-mediated transcriptional regulation. ELP5 has some isoforms with MW 20 kDa, 30 kDa and 34-37 kDa.

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