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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTC1 Polyclonal antibody (28268-1-AP)

    WB analysis using 28268-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28268-1-AP (C17orf68 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CTC1 Polyclonal antibody (28268-1-AP)

    WB analysis of HeLa using 28268-1-AP

    WB result of C17orf68 antibody (28268-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C17orf68 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CTC1 Polyclonal antibody (28268-1-AP)

    IF Staining of mouse brain using 28268-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CTC1 antibody (28268-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

CTC1 Polyclonal antibody

CTC1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 28268-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, ELISA

Synonyms

C17orf68, AAF 132, CST complex subunit CTC1, HBV DNAPTP1-transactivated protein B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, K562 cells
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28268-1-AP targets CTC1 in WB, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28167

Product name: Recombinant human C17orf68 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 337-566 aa of BC111783

Sequence: PSNSEDKKDPESLVRYSRLLSYSGAVTGVLNEPAGLYELDGQLGLCLAYQQFRGLRRVMRPGVCLQLQDVHLLQSVGGGTRRPVLAPCLRGAVLLQSFSRQKPGAHSSRQAYGASLYEQLVWERQLGLPLYLWATKALEELACKLCPHVLRHHQFLQHSSPGSPSLGLQLLAPTLDLLAPPGSPVRNAHNEILEEPHHCPLQKYTRLQTPSSFPTLATLKEEGQRKAWAS

相同性解析による交差性が予測される生物種
Full Name chromosome 17 open reading frame 68
Calculated molecular weight 1217 aa, 135 kDa
Observed molecular weight 135-150 kDa
GenBank accession numberBC111783
Gene Symbol CTC1
Gene ID (NCBI) 80169
RRIDAB_2918152
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2NKJ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Conserved telomere maintenance component 1 (CTC1) is a subunit of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase, the enzyme that initiates DNA replication (PMID: 19119139). CTC1 also functions in a telomere-associated complex with STN1 and TEN1 (PMID: 19854130).

Protocols

Product Specific Protocols
IF protocol for CTC1 antibody 28268-1-APDownload protocol
WB protocol for CTC1 antibody 28268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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