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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C17orf37 Polyclonal antibody (20876-1-AP)

    WB analysis using 20876-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20876-1-AP (C17orf37 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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MIEN1 Polyclonal antibody

MIEN1 Polyclonal Antibody for WB, ELISA
Cat No. 20876-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C17orf37, C35, HBV XAg-transactivated protein 4, HBV X-transactivated gene 4 protein, Migration and invasion enhancer 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inA549 cells, DU 145 cells, SK-BR-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20876-1-AP targets MIEN1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIEN1 fusion protein Ag14975 相同性解析による交差性が予測される生物種
Full Name chromosome 17 open reading frame 37
Calculated molecular weight 115 aa, 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC006006
Gene Symbol C17orf37
Gene ID (NCBI) 84299
RRIDAB_3085626
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRT3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C17orf37 (chromosome 17 open reading frame 37), also known as C35/Rdx12/MGC14832, is located on human chromosome 17q12, 505 nucleotides from the 3¶ end of the ERBB2 oncogenes. C17orf37 expression positively correlates with grade and stage of breast cancer, and increased expression has been shown in prostate cancer cells and tissue specimens (PMID: 17121940, 19503095)..C17orf37 gene encodes a 12-kDa protein that does not have sequence similarity with any known protein. C17orf37 is expressed as a cytosolic protein with predominant membrane localization (PMID: 21628459).

Protocols

Product Specific Protocols
WB protocol for MIEN1 antibody 20876-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols