Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, MOLT-4 cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26923-1-AP targets VPS35L in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VPS35L fusion protein Ag25620 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 16 open reading frame 62 |
| Observed molecular weight | 98 kDa |
| GenBank accession number | BC058845 |
| Gene Symbol | C16orf62 |
| Gene ID (NCBI) | 57020 |
| RRID | AB_3669574 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z3J2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VPS35L, also known as EC97, contains 5 N-glycosylation sites, 3 N-myristoylation sites, and numerous phosphorylation sites. Northern blot analysis detected a 3.4-kb EC97 transcript in all human tissues tested except adipose. RT-PCR analysis showed that EC97 expression was upregulated in 60% of human esophageal squamous cell carcinoma (ESCC) tissues and downregulated in 35% of ESCC tissues tested compared with controls. VPS35L is Involved in Golgi to plasma membrane transport and endocytic recycling.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS35L antibody 26923-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |