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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C16orf14 Polyclonal antibody (20808-1-AP)

    WB analysis using 20808-1-AP (same clone as 20808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20808-1-AP (C16orf14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20808-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using C16orf14 Polyclonal antibody (20808-1-AP)

    WB analysis of mouse skin using 20808-1-AP (same clone as 20808-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 20808-1-AP (C16orf14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20808-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using C16orf14 Polyclonal antibody (20808-1-AP)

    WB analysis of mouse skin using 20808-1-AP (same clone as 20808-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 20808-1-AP (C16orf14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using C16orf14 Polyclonal antibody (20808-1-AP)

    IHC staining of mouse heart using 20808-1-AP (same clone as 20808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20808-1-AP (C16orf14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using C16orf14 Polyclonal antibody (20808-1-AP)

    IHC staining of mouse heart using 20808-1-AP (same clone as 20808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20808-1-AP (C16orf14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20808-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C16orf14 Polyclonal antibody (20808-1-AP)

    IF Staining of HepG2 using 20808-1-AP (same clone as 20808-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C16orf14 antibody (20808-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20808-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C16orf14 Polyclonal antibody (20808-1-AP)

    IF Staining of HepG2 using 20808-1-AP (same clone as 20808-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using C16orf14 antibody 20808-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20808-1-PBS in a different storage buffer formulation.

C16orf14 Polyclonal antibody, PBS Only

C16orf14 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20808-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

c349E10.1, FAM195A, MAPK regulated corepressor interacting protein 2, MCRIP2, Protein FAM195A

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20808-1-PBS targets C16orf14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14754

Product name: Recombinant human C16orf14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-75 aa of BC007346

Sequence: MYTITKGPSKLVAQRRTGPTQQQVEGRLGELLKCRQPAPPTSQPPRAQPFAQPPGPWPLSSLAAGATAAGWWPSR

相同性解析による交差性が予測される生物種
Full Name chromosome 16 open reading frame 14
Calculated molecular weight 160 aa, 18 kDa
Observed molecular weight 8 kDa, 18 kDa
GenBank accession numberBC007346
Gene Symbol C16orf14
Gene ID (NCBI) 84331
RRIDAB_10700013
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUT9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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