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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C15orf55 Polyclonal antibody (28639-1-AP)

    WB analysis using 28639-1-AP (same clone as 28639-1-PBS)

    Mouse liver tissue were subjected to SDS PAGE followed by western blot with 28639-1-AP (C15orf55 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28639-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C15orf55 Polyclonal antibody (28639-1-AP)

    IHC staining of mouse testis using 28639-1-AP (same clone as 28639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28639-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using C15orf55 Polyclonal antibody (28639-1-AP)

    IHC staining of mouse skin using 28639-1-AP (same clone as 28639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28639-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using C15orf55 Polyclonal antibody (28639-1-AP)

    IHC staining of mouse skin using 28639-1-AP (same clone as 28639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28639-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using C15orf55 Polyclonal antibody (28639-1-AP)

    IHC staining of mouse skin using 28639-1-AP (same clone as 28639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28639-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C15orf55 Polyclonal antibody (28639-1-AP)

    IHC staining of mouse testis using 28639-1-AP (same clone as 28639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28639-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C15orf55 Polyclonal antibody (28639-1-AP)

    IF Staining of A431 using 28639-1-AP (same clone as 28639-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C15orf55 antibody (28639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28639-1-PBS in a different storage buffer formulation.

C15orf55 Polyclonal antibody, PBS Only

C15orf55 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28639-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28639-1-PBS targets C15orf55 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29239

Product name: Recombinant human C15orf55 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 496-783 aa of BC114567

Sequence: SGSVEDEDGDGRLRPSPGLQGAGGAACLGKVSSSGKRAREVHGGQEQALDSPRGMHRDGNTLPSPSSWDLQPELAAPQGTPGPLGVERRGSGKVINQVSLHQDGHLGGAGPPGHCLVADRTSEALPLCWQGGFQPESTPSLDAGLAELAPLQGQGLEKQVLGLQKGQQTGGRGVLPQGKEPLAVPWEGSSGAMWGDDRGTPMAQSYDQNPSPRAAGERDDVCLSPGVWLSSEMDAVGLELPVQIEEVIESFQVEKCVTEYQEGCQGLGSRGNISLGPGETLVPGDTES

相同性解析による交差性が予測される生物種
Full Name chromosome 15 open reading frame 55
Calculated molecular weight 1132 aa, 120 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC114567
Gene Symbol C15orf55
Gene ID (NCBI) 256646
RRIDAB_2881186
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86Y26
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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