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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C14orf126 Polyclonal antibody (23923-1-AP)

    WB analysis using 23923-1-AP (same clone as 23923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23923-1-AP (C14orf126 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23923-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using C14orf126 Polyclonal antibody (23923-1-AP)

    WB analysis using 23923-1-AP (same clone as 23923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23923-1-AP (C14orf126 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using C14orf126 Polyclonal antibody (23923-1-AP)

    IHC staining of mouse lung using 23923-1-AP (same clone as 23923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 23923-1-AP (C14orf126 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23923-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using C14orf126 Polyclonal antibody (23923-1-AP)

    IF Staining of A549 using 23923-1-AP (same clone as 23923-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C14orf126 antibody (23923-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23923-1-PBS in a different storage buffer formulation.

C14orf126 Polyclonal antibody, PBS Only

C14orf126 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23923-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Animalia-specific tRNA deacylase, ATD, D-aminoacyl-tRNA deacylase 2, DTD2, D-tyrosyl-tRNA(Tyr) deacylase 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23923-1-PBS targets C14orf126 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21053

Product name: Recombinant human C14orf126 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-168 aa of BC010618

Sequence: MAEGSRIPQARALLQQCLHARLQIRPADGDVAAQWVEVQRGLVIYVCFFKGADKELLPKMVNTLLNVKLSETENGKHVSILDLPGNILIIPQATLGGRLKGRNMQYHSNSGKEEGFELYSQFVTLCEKEVAANSKCAEARVVVEHGTYGNRQVLKLDTNGPFTHLIEF

相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 126
Calculated molecular weight 168 aa, 19 kDa
Observed molecular weight19 kDa
GenBank accession numberBC010618
Gene Symbol C14orf126
Gene ID (NCBI) 112487
RRIDAB_3669426
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96FN9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C14orf126, also named D-aminoacyl-tRNA deacylase 2 (DTD2), can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Plants are unique in possessing two distinct chiral proofreading systems, D-aminoacyl-tRNA deacylase1 (DTD1) and DTD2, of bacterial and archaeal origins, respectively.

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