Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IP | See 3 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
24968-1-AP targets NRDE2 in WB, IHC, IP, CoIP, chIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21805 Product name: Recombinant human C14orf102 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 615-933 aa of BC118673 Sequence: AVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQSCPWAKVLYLDAVEYFPDEMQEILDLMTEKELRVRLPLEELELLLED 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 14 open reading frame 102 |
| Calculated molecular weight | 1164 aa, 133 kDa |
| Observed molecular weight | 133 kDa |
| GenBank accession number | BC118673 |
| Gene Symbol | NRDE2 |
| Gene ID (NCBI) | 55051 |
| RRID | AB_2879825 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H7Z3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for NRDE2 antibody 24968-1-AP | Download protocol |
| WB protocol for NRDE2 antibody 24968-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genes Dev An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. | ||
RNA Biol NRDE-2, the human homolog of fission yeast Nrl1, prevents DNA damage accumulation in human cells. | ||
J Virol Kaposi's Sarcoma-Associated Herpesvirus Fine-Tunes the Temporal Expression of Late Genes by Manipulating a Host RNA Quality Control Pathway.
| ||
Cell Genom NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors
| ||
Genes Dev NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.
|
