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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C12orf51 Polyclonal antibody (20609-1-AP)

    WB analysis using 20609-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20609-1-AP (C12orf51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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C12orf51 Polyclonal antibody

C12orf51 Polyclonal Antibody for WB, ELISA
Cat No. 20609-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

HECTD4, Probable E3 ubiquitin-protein ligase HECTD4, KIAA0614, HECT-type E3 ubiquitin transferase HECTD4, HECT domain-containing protein 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20609-1-AP targets C12orf51 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C12orf51 fusion protein Ag14614 相同性解析による交差性が予測される生物種
Full Name chromosome 12 open reading frame 51
Calculated molecular weight 3996 aa, 439 kDa
Observed molecular weight500 kDa
GenBank accession numberBC006270
Gene Symbol C12orf51
Gene ID (NCBI) 283450
RRIDAB_3669337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The HECT domain E3 ubiquitin protein ligase 4 (HECTD4), is also known as C12orf51. It may be involved in protein modification or ubiquitination (PMID: 26891264). In addition, genetic polymorphisms of C12orf51 are related to alcohol consumption and type 2 diabetes (T2D) (PMID: 38523829).

Protocols

Product Specific Protocols
WB protocol for C12orf51 antibody 20609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols