Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells, ACHN cells, Calu-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33971-1-AP targets C12orf43 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40576 Product name: Recombinant human C12orf43 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 33-142 aa of BC014661 Sequence: AWGLEQRPHVAGKPRAGAANSQLSTSQPSLRHKVNEHEQDGNELQTTPEFRAHVAKKLGALLDSFITISEAAKEPAKAKVQKVALEDDGFRLFFTSVPGGREKEESPQPR 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 12 open reading frame 43 |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC014661 |
| Gene Symbol | C12orf43 |
| Gene ID (NCBI) | 64897 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96C57 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C12orf43 (also known as CUSTOS) is a nuclear-localized protein. It is predicted to be involved in the formation of the Spemann organizer, a key process in embryonic development, and acts as a negative regulator of the Wnt signaling pathway. Studies have shown that C12orf43 is differentially expressed in various tumors, and its high expression in liver cancer and lung cancer is significantly associated with poor patient prognosis, establishing it as an unfavorable prognostic marker. Additionally, C12orf43 has been found to be associated with neurodegenerative diseases, autoimmune disorders, and other conditions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C12orf43 antibody 33971-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |