Validation Data Gallery
Tested Applications
| Positive WB detected in | Caco-2 cells, K-562 cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33680-1-AP targets RESF1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38168 Product name: Recombinant human C12orf35 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1399-1747 aa of BC140819 Sequence: GSVGATFKLGDSLSNPNERAIVKEKMVSNTKSVDTKASSSKFSRILTPKEYLQRQKHKEALSNKASKKICVKNVPCDSEHMRPSKLAVQVESCGKSNEKHSSGVQTSKESLNGLTSHGKNLKIHHSQESKTYNILRNVKEKVGGKQPDKIWIDKTKLDKLTNISNEAQFSQMPPQVKDQKKLYLNRVGFKCTERESISLTKLESSPRKLHKDKRQENKHKTFLPVKGNTEKSNMLEFKLCPDILLKNTNSVEERKDVKPHPRKEQAPLQVSGIKSTKEDWLKFVATKKRTQKDSQERDNVNSRLSKRSFSADGFEMLQNPVKDSKEMFQTYKQMYLEKRSRSLGSSPVK 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 12 open reading frame 35 |
| Calculated molecular weight | 1747 aa, 195 kDa |
| Observed molecular weight | 240-250 kDa |
| GenBank accession number | BC140819 |
| Gene Symbol | C12orf35 |
| Gene ID (NCBI) | 55196 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9HCM1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RESF1 (Retroelement Silencing Factor 1, also known as C12orf35 or KIAA1551) is a nuclear protein that primarily mediates the epigenetic silencing of endogenous retroviruses (ERVs) and imprinted genes by interacting with the SETDB1 histone methyltransferase, thereby maintaining H3K9me3 modifications and heterochromatin conformation. Recent studies have revealed that RESF1 exerts metastasis-suppressive functions in estrogen receptor-negative breast cancer. Additionally, RESF1 negatively regulates ribosomal biogenesis and global protein translation, serving as a multifunctional factor that links genome stability, stem cell pluripotency, and tumor suppression.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RESF1 antibody 33680-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |