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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C12orf29 Polyclonal antibody (31647-1-AP)

    WB analysis using 31647-1-AP (same clone as 31647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31647-1-AP (C12orf29 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using C12orf29 Polyclonal antibody (31647-1-AP)

    WB analysis of HeLa using 31647-1-AP (same clone as 31647-1-PBS)

    WB result of C12orf29 antibody (31647-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C12orf29 transfected HeLa cells. This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using C12orf29 Polyclonal antibody (31647-1-AP)

    IHC staining of mouse small intestine using 31647-1-AP (same clone as 31647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31647-1-AP (C12orf29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using C12orf29 Polyclonal antibody (31647-1-AP)

    IHC staining of mouse small intestine using 31647-1-AP (same clone as 31647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31647-1-AP (C12orf29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C12orf29 Polyclonal antibody (31647-1-AP)

    IF Staining of HeLa using 31647-1-AP (same clone as 31647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C12orf29 antibody (31647-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

C12orf29 Polyclonal antibody, PBS Only

C12orf29 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31647-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RNA Ligase, RNA Ligase 1, Rnl

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31647-1-PBS targets C12orf29 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35681

Product name: Recombinant human C12orf29 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of NM_001009894.2

Sequence: MKRLGSVQRKMPCVFVTEVKEEPSSKREHQPFKVLATETVSHKALDADIYSAIPTEKVDGTCCYVTTYKDQPYLWARLDRKPNKQAEKRFKNFLHSKENPKEFFWNVEEDFKPAPECWIPAKETEQINGNPVPDENGHIPGWVPVEKNNK

相同性解析による交差性が予測される生物種
Full Name chromosome 12 open reading frame 29
Calculated molecular weight37kDa，325aa
Observed molecular weight37 kDa
GenBank accession numberNM_001009894.2
Gene Symbol C12orf29
Gene ID (NCBI) 91298
RRIDAB_3670063
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8N999
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).

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