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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using C11orf68 Recombinant antibody (84725-5-RR)

    WB analysis of A431 using 84725-5-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 84725-5-RR (C11orf68 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using C11orf68 Recombinant antibody (84725-5-RR)

    WB analysis of PC-3 using 84725-5-RR

    PC-3 cells were subjected to SDS PAGE followed by western blot with 84725-5-RR (C11orf68 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using C11orf68 Recombinant antibody (84725-5-RR)

    IHC staining of human stomach cancer using 84725-5-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84725-5-RR (C11orf68 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C11orf68 Recombinant antibody (84725-5-RR)

    IF Staining of A431 using 84725-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C11orf68 antibody (84725-5-RR, Clone: 242221C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84725-5-RR

    Affinity and Kinetic Characterization of 84725-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84725-5-RR against Human C11orf68 were performed. The affinity constant is 11.8 nM.

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C11orf68 Recombinant antibody

C11orf68 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84725-5-RR
Clone No.242221C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UPF0696 protein C11orf68, Protein p5326, P5326, BLES03, 242221C11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, PC-3 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84725-5-RR targets C11orf68 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C11orf68 fusion protein Ag36023 相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 68
Observed molecular weight35 kDa
GenBank accession numberBC010512
Gene Symbol C11orf68
Gene ID (NCBI) 83638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H3H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BLES03 (basophilic leukemia-expressed protein 03) is a human protein of unknown function. Recent studies have shown that BLES03 is a novel eukaryotic phosphothreonine cleavage enzyme involved in the biosynthesis of dehydrogenated amino acids.The crystal structure of BLES03 has been resolved by single-wavelength anomalous scattering (SAD) with a resolution of 2.5 Å.

Protocols

Product Specific Protocols
WB protocol for C11orf68 antibody 84725-5-RRDownload protocol
IHC protocol for C11orf68 antibody 84725-5-RRDownload protocol
IF protocol for C11orf68 antibody 84725-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols