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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC15 Polyclonal antibody

APC15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20409-1-AP

Synonyms

APC15, C11orf51, HSPC020

Validation Data Gallery

Applications:
  • WB analysis of HT-29 using 20409-1-AP

    WB analysis of HT-29 using 20409-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 20409-1-AP

    WB analysis of HEK-293 using 20409-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 20409-1-AP

    IHC staining of human lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 20409-1-AP

    IHC staining of human lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human pancreas using 20409-1-AP

    IHC staining of human pancreas using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 20409-1-AP

    IHC staining of human pancreas using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHT-29 cells, HEK-293 cells
Positive IHC detected inhuman lung tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20409-1-AP is APC15 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APC15 fusion protein Ag14237 相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 51
Calculated molecular weight 121 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC005156
Gene symbol C11orf51
Gene ID (NCBI) 25906
RRIDAB_10694279
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APC15 antibody 20409-1-APDownload protocol
IHC protocol for APC15 antibody 20409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols