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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using C11orf42 Polyclonal antibody (20919-1-AP)

    WB analysis of MCF-7 using 20919-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20919-1-AP (C11orf42 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using C11orf42 Polyclonal antibody (20919-1-AP)

    IF Staining of MCF-7 using 20919-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20919-1-AP (C11orf42 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using C11orf42 Polyclonal antibody (20919-1-AP)

    IF Staining of MCF-7 using 20919-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using C11orf42 antibody (20919-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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C11orf42 Polyclonal antibody

C11orf42 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20919-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20919-1-AP targets C11orf42 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C11orf42 fusion protein Ag15118 相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 42
Calculated molecular weight 333 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC031612
Gene Symbol C11orf42
Gene ID (NCBI) 160298
RRIDAB_2878764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8N5U0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C11orf42 antibody 20919-1-APDownload protocol
IF protocol for C11orf42 antibody 20919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols