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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using C10orf68 Polyclonal antibody (24975-1-AP)

    WB analysis of A549 using 24975-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24975-1-AP (C10orf68 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COS-7 cells using C10orf68 Polyclonal antibody (24975-1-AP)

    WB analysis of COS-7 using 24975-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 24975-1-AP (C10orf68 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human kidney tissue using C10orf68 Polyclonal antibody (24975-1-AP)

    IHC staining of human kidney using 24975-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24975-1-AP (C10orf68 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C10orf68 Polyclonal antibody (24975-1-AP)

    IHC staining of human kidney using 24975-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24975-1-AP (C10orf68 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C10orf68 Polyclonal antibody (24975-1-AP)

    IF Staining of HEK-293 using 24975-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24975-1-AP (C10orf68 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C10orf68 Polyclonal antibody (24975-1-AP)

    IF Staining of HEK-293 using 24975-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using C10orf68 antibody (24975-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C10orf68 Polyclonal antibody (24975-1-AP)

    IF Staining of HEK-293 using 24975-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24975-1-AP (C10orf68 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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C10orf68 Polyclonal antibody

C10orf68 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24975-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein BIOT2, Coiled-coil domain-containing protein 7, CCDC7, BIOT2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, COS-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24975-1-AP targets C10orf68 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, monkey samples.

Tested Reactivity human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C10orf68 fusion protein Ag21682 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 68
Calculated molecular weight 628 aa, 71 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC125091
Gene Symbol C10orf68
Gene ID (NCBI) 79741
RRIDAB_2879828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

chromosome 10 open reading frame 68 (C10orf68) is a 628 amino-acid protein, which may have three isoforms. The function of C10orf68 is still non-known and needs to be further studied.

Protocols

Product Specific Protocols
WB protocol for C10orf68 antibody 24975-1-APDownload protocol
IHC protocol for C10orf68 antibody 24975-1-APDownload protocol
IF protocol for C10orf68 antibody 24975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols