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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using C10orf67 Polyclonal antibody (24459-1-AP)

    WB analysis of PC-3 using 24459-1-AP (same clone as 24459-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24459-1-AP (C10orf67 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24459-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using C10orf67 Polyclonal antibody (24459-1-AP)

    WB analysis of human testis using 24459-1-AP (same clone as 24459-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 24459-1-AP (C10orf67 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24459-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using C10orf67 Polyclonal antibody (24459-1-AP)

    WB analysis of A549 using 24459-1-AP (same clone as 24459-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 24459-1-AP (C10orf67 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24459-1-PBS in a different storage buffer formulation.

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C10orf67 Polyclonal antibody, PBS Only

C10orf67 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 24459-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24459-1-PBS targets C10orf67 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C10orf67 fusion protein Ag21465 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 67
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC035732
Gene Symbol C10orf67
Gene ID (NCBI) 256815
RRIDAB_2879558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYJ2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RNF17 also termed as ring finger protein 17 is a 1623 amino acid protein, which contains 1 ring type zinc finger and 4 tudor domains. RNF17 is specifically expressed in testis. The RING finger motif presents in many ubiquitin E3 ligases. RNF17 interacts with all four members of the Mad family (Mad1, Mxi1, Mad3 and Mad4), which are basic-helix-loop-helix-leucine zipper transcription factors of the Myc oncoprotein network. RNF17 is component of the tectonic-like complex, which is required for ciliogenesis and sonic hedgehog/SHH signaling. RNF17 is able to form dimers or polymers both in vitro and in vivo, indicating that it may play a role in the assembly of RNF17 granules. RNF17 encodes a novel key regulator of spermiogenesis.