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Validation Data Gallery

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  • Western Blot (WB) analysis of ROS1728 cells using C10orf58 Polyclonal antibody (20446-1-AP)

    WB analysis of ROS1728 using 20446-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 20446-1-AP (C10orf58 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using C10orf58 Polyclonal antibody (20446-1-AP)

    IHC staining of human skin using 20446-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using C10orf58 Polyclonal antibody (20446-1-AP)

    IHC staining of human skin using 20446-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of ROS1728 cells using C10orf58 Polyclonal antibody (20446-1-AP)

    IF Staining of ROS1728 using 20446-1-AP

    Immunofluorescent analysis of ROS1728 cells using 20446-1-AP (C10orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of ROS1728 cells using C10orf58 Polyclonal antibody (20446-1-AP)

    IF Staining of ROS1728 using 20446-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed ROS1728 cells using C10orf58 antibody (20446-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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C10orf58 Polyclonal antibody

C10orf58 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20446-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

F213A, FAM213A, PAMM, Peroxiredoxin-like 2 activated in M-CSF stimulated monocytes, Peroxiredoxin-like 2A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inROS1728 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inROS1728 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

20446-1-AP targets C10orf58 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C10orf58 fusion protein Ag14272 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 58
Calculated molecular weight 229 aa, 26 kDa
Observed molecular weight 24-30 kDa
GenBank accession numberBC005871
Gene Symbol C10orf58
Gene ID (NCBI) 84293
RRIDAB_2878687
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C10orf58, also name as FAM213A and PAMM, is involved in redox regulation of the cell. It acts as an antioxidant. C10orf58 inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation. It may affect bone resorption and help to maintain bone mass. The MW of C10orf58 is 24-30 kDa.

Protocols

Product Specific Protocols
WB protocol for C10orf58 antibody 20446-1-APDownload protocol
IHC protocol for C10orf58 antibody 20446-1-APDownload protocol
IF protocol for C10orf58 antibody 20446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
HumanIHC

Oncol Rep

Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1

Authors - Jie Zhang
View Article