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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C10orf28 Polyclonal antibody

C10orf28 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24967-1-AP

Synonyms

C10orf28, C10orf28 protein, GIDRP86, PSORT

Validation Data Gallery

Applications:
  • WB analysis of A549 using 24967-1-AP

    WB analysis of A549 using 24967-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24967-1-AP (C10orf28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 24967-1-AP

    WB analysis of A431 using 24967-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 24967-1-AP (C10orf28 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IHC staining of human small intestine using 24967-1-AP

    IHC staining of human small intestine using 24967-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24967-1-AP (C10orf28 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human small intestine using 24967-1-AP

    IHC staining of human small intestine using 24967-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24967-1-AP (C10orf28 Antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 24967-1-AP

    IF Staining of HepG2 using 24967-1-AP

    Immunofluorescent analysis of HepG2 cells using 24967-1-AP (C10orf28 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, A431 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24967-1-AP is C10orf28 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C10orf28 fusion protein Ag21740 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 28
Calculated molecular weight 792 aa, 88 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC098359
Gene symbol C10orf28
Gene ID (NCBI) 27291
RRIDAB_2879824
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C10orf28 antibody 24967-1-APDownload protocol
IHC protocol for C10orf28 antibody 24967-1-APDownload protocol
IF protocol for C10orf28 antibody 24967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols