Validation Data Gallery
Tested Applications
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26324-1-AP targets C10orf104 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23641 Product name: Recombinant human C10orf104 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-110 aa of BC009530 Sequence: MAASSSSSSAGGVSGSSVTGSGFSVSDLAPPRKALFTYPKGAGEMLEDGSERFLCESVFSYQVASTLKQVKHDQQVARMEKLAGLVEELEADEWRFKPIEQLLGFTPSSG 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 10 open reading frame 104 |
| Observed molecular weight | 12 kDa |
| GenBank accession number | BC009530 |
| Gene Symbol | C10orf104 |
| Gene ID (NCBI) | 119504 |
| RRID | AB_3742181 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96DE5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C10orf104 (also known as ANAPC16 or APC16) is a protein associated with the anaphase-promoting complex/cyclosome (APC/C). APC/C is a highly conserved E3 ubiquitin ligase that regulates various stages of the cell cycle, particularly the transition from mitotic to non-mitotic phases and the entry into the G1 phase during mitosis. As a subunit of APC/C, C10orf104 contributes to its stability and modulates its ubiquitin ligase activity.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for C10orf104 antibody 26324-1-AP | Download protocol |
| IHC protocol for C10orf104 antibody 26324-1-AP | Download protocol |
| IP protocol for C10orf104 antibody 26324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |