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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293 cells using C10orf104 Polyclonal antibody (26324-1-AP)

    IP experiment of HEK-293 using 26324-1-AP

    IP result of anti-C10orf104 (IP:26324-1-AP, 4ug; Detection:26324-1-AP 1:800) with HEK-293 cells lysate 1440 ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using C10orf104 Polyclonal antibody (26324-1-AP)

    IHC staining of human stomach cancer using 26324-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26324-1-AP (C10orf104 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C10orf104 Polyclonal antibody (26324-1-AP)

    IF Staining of A431 using 26324-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using C10orf104 antibody (26324-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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C10orf104 Polyclonal antibody

C10orf104 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 26324-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, ELISA

Synonyms

ANAPC16, Anaphase-promoting complex subunit 16, APC16, CENP-27, Cyclosome subunit 16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26324-1-AP targets C10orf104 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C10orf104 fusion protein Ag23641 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 104
Observed molecular weight12 kDa
GenBank accession numberBC009530
Gene Symbol C10orf104
Gene ID (NCBI) 119504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C10orf104 (also known as ANAPC16 or APC16) is a protein associated with the anaphase-promoting complex/cyclosome (APC/C). APC/C is a highly conserved E3 ubiquitin ligase that regulates various stages of the cell cycle, particularly the transition from mitotic to non-mitotic phases and the entry into the G1 phase during mitosis. As a subunit of APC/C, C10orf104 contributes to its stability and modulates its ubiquitin ligase activity.

Protocols

Product Specific Protocols
IHC protocol for C10orf104 antibody 26324-1-APDownload protocol
IF protocol for C10orf104 antibody 26324-1-APDownload protocol
IP protocol for C10orf104 antibody 26324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols