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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using C-Reactive Protein/CRP Recombinant antibody (83741-3-RR)

    WB analysis of rat liver using 83741-3-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat liver tissue using C-Reactive Protein/CRP Recombinant antibody (83741-3-RR)

    IHC staining of rat liver using 83741-3-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using C-Reactive Protein/CRP Recombinant antibody (83741-3-RR)

    IHC staining of rat liver using 83741-3-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83741-3-RR

    Affinity and Kinetic Characterization of 83741-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83741-3-RR against Rat Crp were performed. The affinity constant is below 1 pM.

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C-Reactive Protein/CRP Recombinant antibody

C-Reactive Protein/CRP Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83741-3-RR
Clone No.240759B8

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, ELISA

Synonyms

PTX1, PTX 1, CRP, 240759B8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83741-3-RR targets C-Reactive Protein/CRP in WB, IHC, ELISA applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name C-reactive protein, pentraxin-related
Calculated molecular weight25 kDa
Observed molecular weight 25 kDa
GenBank accession numberNM_017096.3
Gene Symbol Crp
Gene ID (NCBI) 25419
RRIDAB_3671339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP48199
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein belonging to the pentraxin family. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Protocols

Product Specific Protocols
WB protocol for C-Reactive Protein/CRP antibody 83741-3-RRDownload protocol
IHC protocol for C-Reactive Protein/CRP antibody 83741-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols