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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Broad Range Prestained Protein Marker

Molecular Weight

3-245 kDa

Detection Method

Colorimetric, Fluorescence

Number of Markers

13

Stain Type

3 colors: Blue, Red, Green

Cat no : PL00002

Validation Data Gallery

  • Resolution of the Broad Range Prestained Protein Ladder in a 4-12% Bis-Tris gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

    Resolution of the Broad Range Prestained Protein Ladder in a 4-12% Bis-Tris gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

  • <strong>Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions</strong><br>The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.

    Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions
    The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.

  • The prestained protein ladders were resolved in an 8-16% Tris-glycine gel (SDS-PAGE). The image shows a comparison of the migration patterns on the gel for our standard (PL00001), broad (PL00002), and extra broad (PL00003) range ladders.

    The prestained protein ladders were resolved in an 8-16% Tris-glycine gel (SDS-PAGE). The image shows a comparison of the migration patterns on the gel for our standard (PL00001), broad (PL00002), and extra broad (PL00003) range ladders.

  • 2 uL protein ladders were loaded in the 8%-18% Tris-glycine gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75 kDa and 310 kDa) and 680 nm (except 75 kDa and 310 kDa) range.

    2 uL protein ladders were loaded in the 8%-18% Tris-glycine gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75 kDa and 310 kDa) and 680 nm (except 75 kDa and 310 kDa) range.

Product Information

The PL00002 Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 prestained proteins covering a wide range of molecular weights from 3 to 245 kDa in Tris-Glycine buffer. The PL00002 Broad Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins. The PL00002 Broad Range Protein Marker is also suitable for fluorescence WB detection.

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Storage

Store product at 4℃ for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.

Usage

The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Detection Method

Colorimetric

Molecular Weight

~ 3, 10, 15, 20, 25, 35, 45, 60, 75, 100, 140, 180, 245 kDa

Number of Markers

13

Size Range

3 to 245

Stain Type

3 colors: Blue, Red, Green

Cited in Article as

pl00002, Broad Range Prestained Protein Marker, Proteintech, IL, USA

Documentation

SDS
Prestained_Potein Marker 3-245kDa SDS

Publications

ApplicationTitle

iScience

Spatiotemporal dynamics exhibited by horizontal basal cells reveal a pro-neurogenic pathway during injury-induced olfactory epithelium regeneration

Authors - Jonathan D Louie
View Article

Water Res

Mechanistic insights into chloroethene dechlorination by Dehalococcoides mccartyi strain CWV2: A multi-omics perspective

Authors - Chih-Ming Kao
View Article