Broad Range Prestained Protein Marker

Molecular Weight

3-245 kDa

Detection Method

Colorimetric

Number of Markers

13

Stain Type

3 colors: Blue, Red, Green

Cat no : PL00002



Product Information

The PL00002 Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 prestained proteins covering a wide range of molecular weights from 3 to 245 kDa in Tris-Glycine buffer. The PL00002 Broad Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins.

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Storage

Store product at 4 for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.

Usage

The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Detection Method

Colorimetric

Molecular Weight

~ 3, 10, 15, 20, 25, 35, 45, 60, 75, 100, 140, 180, 245 kDa

Number of Markers

13

Size Range

3 to 245

Stain Type

3 colors: Blue, Red, Green

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christine (Verified Customer) (09-19-2023)

Very good protein and economical marker, same as PL00001 but with a few extra bands. 1 ul loaded on a SDS PAGE gel and transferred to nitrocellulose are enough for a clear detection of all the sizes. Of note: the pink marker (75 kDa) is not detected in the LICOR IR680 or IR800 channels but all others are OK.

  • Applications: Western Blot
FH

Andrea (Verified Customer) (12-03-2022)

A good, strong signal is provided by loading a few microliters of the product.

  • Applications: Western Blot
FH

Dan (Verified Customer) (09-30-2022)

Low price but you get a very good quality of protein ladder!

  • Applications: Western Blot,Protein purification
FH

Mary (Verified Customer) (09-19-2022)

Worked okay. It's a decent protein marker.

  • Applications: Western Blot
FH

Cloure (Verified Customer) (08-25-2022)

Worked pretty decently

  • Applications: Western Blot
  • Cell Tissue Type: Testicle
FH

Mickael (Verified Customer) (08-15-2022)

Worked like a charm!

  • Applications: Western Blot
FH

Maryna (Verified Customer) (08-13-2022)

Was very happy with the results, will ask my PI for to change

  • Applications: Western Blot
FH

Francisco (Verified Customer) (08-13-2022)

Performed very well in a pilot gel!

  • Applications: Western Blot
  • Cell Tissue Type: Spleen
FH

Miguel (Verified Customer) (08-09-2022)

Very nice, will be switching when starting new project

  • Applications: Western Blot
FH

Miguel (Verified Customer) (08-05-2022)

It worked great.

  • Applications: Western Blot
FH

Duncan (Verified Customer) (10-20-2021)

Perfect for all my Western blotting

  • Applications: Western Blot
FH

Adrian (Verified Customer) (09-02-2021)

Protein ladder we obtained from colleagues from Biochemistry worked great and exhibited clear, distinguishable bands. Good overall quality for research on food allergenicity. Would highly recommend.

  • Applications: Western Blot
  • Cell Tissue Type: N/A
FH

Maria (Verified Customer) (08-23-2021)

Cell lysate 10ug labelled with No-stain (590 nm emission). 4-15% TGX gel. PL00002 5 uL on the left. Very good marker.

  • Applications: Western Blot
FH

Phoebe (Verified Customer) (03-10-2020)

This ladder worked brilliantly on my western. I have a large protein so need a broader-range ladder, for which I have previously been using PageRuler Plus. The new Proteintech ladder has been a very good replacement.

  • Applications: Western Blot,