Product Information
The PL00002 Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 prestained proteins covering a wide range of molecular weights from 3 to 245 kDa in Tris-Glycine buffer. The PL00002 Broad Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins. The PL00002 Broad Range Protein Marker is also suitable for fluorescence WB detection.
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Storage
Store product at 4℃ for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.
Usage
The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.
Detection Method
Colorimetric
Molecular Weight
~ 3, 10, 15, 20, 25, 35, 45, 60, 75, 100, 140, 180, 245 kDa
Number of Markers
13
Size Range
3 to 245
Stain Type
3 colors: Blue, Red, Green
Cited in Article as
pl00002, Broad Range Prestained Protein Marker, Proteintech, IL, USA
Documentation
| SDS |
|---|
| Prestained_Potein Marker 3-245kDa SDS |
| Datasheet |
|---|
| Broad Range Prestained Protein Marker PL00002 datasheet |
Publications
| Application | Title |
|---|---|
iScience Spatiotemporal dynamics exhibited by horizontal basal cells reveal a pro-neurogenic pathway during injury-induced olfactory epithelium regeneration | |
Water Res Mechanistic insights into chloroethene dechlorination by Dehalococcoides mccartyi strain CWV2: A multi-omics perspective |