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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of human brain using 19017-1-AP (same clone as 19017-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of SH-SY5Y using 19017-1-AP (same clone as 19017-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of COLO 320 using 19017-1-AP (same clone as 19017-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of Y79 using 19017-1-AP (same clone as 19017-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Brevican Polyclonal antibody (19017-1-AP)

    IHC staining of human cerebellum using 19017-1-AP (same clone as 19017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Brevican Polyclonal antibody (19017-1-AP)

    IHC staining of human cerebellum using 19017-1-AP (same clone as 19017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Brevican Polyclonal antibody (19017-1-AP)

    IHC staining of mouse brain using 19017-1-AP (same clone as 19017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19017-1-AP (Brevican antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

Brevican Polyclonal antibody, PBS Only

Brevican Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19017-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19017-1-PBS targets Brevican in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13552

Product name: Recombinant human BCAN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-350 aa of BC010571

Sequence: LEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPTYPASLTDVSLALNELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVY

相同性解析による交差性が予測される生物種
Full Name brevican
Calculated molecular weight 99 kDa
Observed molecular weight 145 kDa, 72kDa, 80 kDa
GenBank accession numberBC010571
Gene Symbol BCAN
Gene ID (NCBI) 63827
RRIDAB_10643526
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GW7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512). This antibody can recognize the 72 kDa isoform of target.

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