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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using TBXT Polyclonal antibody (20741-1-AP)

    WB analysis of mouse liver using 20741-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20741-1-AP (Brachyury-T antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TBXT Polyclonal antibody (20741-1-AP)

    WB analysis of mouse liver using 20741-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20741-1-AP (Brachyury-T antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

TBXT Polyclonal antibody

TBXT Polyclonal Antibody for WB, ELISA
Cat No. 20741-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Brachyury protein, Brachyury T, Brachyury-T, Protein T, T, brachyury homolog (mouse), T-box transcription factor T, TBXT, TFT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20741-1-AP targets TBXT in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name T, brachyury homolog (mouse)
Calculated molecular weight 47 kDa
Observed molecular weight 44 kDa
GenBank accession numberNM_003181
Gene Symbol TBXT
Gene ID (NCBI) 6862
RRIDAB_2878730
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15178
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Brachyury T is involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. It binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). The antibody is specific to Brachyury T.

Protocols

Product Specific Protocols
WB protocol for TBXT antibody 20741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols