Validation Data Gallery
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Tested Applications
| Positive IHC detected in | mouse skeletal muscle tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 7 publications below |
| IF | See 8 publications below |
Product Information
20277-1-AP targets Osteocalcin in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | bone gamma carboxyglutamate protein 1 |
| Calculated molecular weight | 11 kDa |
| GenBank accession number | NM_007541 |
| Gene Symbol | Osteocalcin |
| Gene ID (NCBI) | 12096 |
| RRID | AB_11232596 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P86546 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Osteocalcin (Bglap1), also named as Bglap2, Bglap, BGP, OC, OG1 and mOC-A, belongs to the osteocalcin/matrix Gla protein family. Bglap1 constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. The antibody is specific to mouse Bglap1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Osteocalcin antibody 20277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Small Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy | ||
J Nanobiotechnology Injectable hydrogels with ROS-triggered drug release enable the co-delivery of antibacterial agent and anti-inflammatory nanoparticle for periodontitis treatment | ||
Acta Biomater A composite membrane with microtopographical morphology to regulate cellular behavior for improved tissue regeneration | ||
Sci Rep Experimental arthritis and Porphyromonas gingivalis administration synergistically decrease bone regeneration in femoral cortical defects. | ||
J Dent Sci Orthodontic tension promotes cementoblast mineralization by regulating autophagy | ||
Phytomedicine Single-cell RNA transcriptomics reveals Du-Zhong-Wan promotes osteoporotic fracture healing via YAP/β-catenin/VEGF axis in BMSCs |