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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Arrestin 2 Monoclonal antibody (68502-1-Ig)

    WB analysis using 68502-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Beta Arrestin 2 Monoclonal antibody (68502-1-Ig)

    WB analysis of K-562 using 68502-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Beta Arrestin 2 Monoclonal antibody (68502-1-Ig)

    WB analysis of NIH/3T3 using 68502-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Beta Arrestin 2 Monoclonal antibody (68502-1-Ig)

    WB analysis of Jurkat using 68502-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Beta Arrestin 2 Monoclonal antibody (68502-1-Ig)

    IHC staining of human colon using 68502-1-Ig

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Beta Arrestin 2 Monoclonal antibody (68502-1-Ig)

    IHC staining of human colon using 68502-1-Ig

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Beta Arrestin 2 Monoclonal antibody (68502-1-Ig)

    IF Staining of HeLa using 68502-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Beta Arrestin 2 antibody (68502-1-Ig, Clone: 1E8F2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

Beta Arrestin 2 Monoclonal antibody

Beta Arrestin 2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68502-1-Ig
Clone No.1E8F2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ARRB2, β-Arrestin 2, BARR2, Arrestin beta-2, ARR2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68502-1-Ig targets Beta Arrestin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29807

Product name: Recombinant human Beta Arrestin 2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 163-409 aa of BC007427

Sequence: NSVRLVIRKVQFAPEKPGPQPSAETTRHFLMSDRSLHLEASLDKELYYHGEPLNVNVHVTNNSTKTVKKIKVSVRQYADICLFSTAQYKCPVAQLEQDDQVSPSSTFCKVYTITPLLSDNREKRGLALDGKLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHIPLPRPQSAAPETDVPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDYDDQLC

相同性解析による交差性が予測される生物種
Full Name arrestin, beta 2
Calculated molecular weight 46 kDa
Observed molecular weight50 kDa
GenBank accession numberBC007427
Gene Symbol Beta Arrestin 2
Gene ID (NCBI) 409
RRIDAB_3085213
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP32121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

Protocols

Product Specific Protocols
IF protocol for Beta Arrestin 2 antibody 68502-1-IgDownload protocol
IHC protocol for Beta Arrestin 2 antibody 68502-1-IgDownload protocol
WB protocol for Beta Arrestin 2 antibody 68502-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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