Validation Data Gallery
Tested Applications
| Positive WB detected in | J774A.1 cells, RAW 264.7 cells, mouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85871-4-RR targets Beta-2-Microglobulin in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3086 Product name: Recombinant Mouse Beta-2-Microglobulin protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 21-119 aa of NM_009735.3 Sequence: IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM 相同性解析による交差性が予測される生物種 |
| Full Name | beta-2 microglobulin |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 12 kDa |
| GenBank accession number | NM_009735.3 |
| Gene Symbol | B2m |
| Gene ID (NCBI) | 12010 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01887 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta-2-Microglobulin antibody 85871-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |