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Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using Bestrophin-1 Monoclonal antibody (60326-1-Ig)

    WB analysis of Y79 using 60326-1-Ig (same clone as 60326-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 60326-1-Ig (Bestrophin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using Bestrophin-1 Monoclonal antibody (60326-1-Ig)

    WB analysis of Transfected HEK-293 using 60326-1-Ig (same clone as 60326-1-PBS)

    WB result of Bestrophin-1 antibody (60326-1-Ig; 1:1000; room temperature for 1.5 hours) with negative control and Bestrophin-1 overexpressed Transfected HEK-293 cells. This data was developed using the same antibody clone with 60326-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Y79 cells using Bestrophin-1 Monoclonal antibody (60326-1-Ig)

    IF Staining of Y79 using 60326-1-Ig (same clone as 60326-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Bestrophin-1 antibody (60326-1-Ig, Clone: 4D5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60326-1-PBS in a different storage buffer formulation.

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Bestrophin-1 Monoclonal antibody, PBS Only

Bestrophin-1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 60326-1-PBS
Clone No.4D5F1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ARB, BEST, BEST1, bestrophin 1, Bestrophin-1, BMD, TU15B, VMD2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60326-1-PBS targets Bestrophin-1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Bestrophin-1 fusion protein Ag15129 相同性解析による交差性が予測される生物種
Full Name bestrophin 1
Calculated molecular weight 585 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC015220
Gene Symbol Bestrophin/BEST1
Gene ID (NCBI) 7439
RRIDAB_2881436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO76090
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Bestrophin-1 (BEST1) is a 68 kDa transmembrane protein which belongs to the bestrophin family of anion channels. It is predominantly expressed in the basolateral membrane of the retinal pigment epithelium. Studies show that bestrophin 1 functions as a Ca(2+)-dependent Cl(-) channel and modulator of voltage-dependent L-type Ca(2+) channels. It may also contribute to the basolateral cell conductance in airway epithelial cells. This protein is encoded by the VMD2 gene. Mutations in VMD2 can lead to different types of retinal or macular degenerations, including Best vitelliform macular dystrophy (BMD), autosomal recessive bestrophinopathy (ARB), autosomal dominant vitreoretinochoroidopathy (ADVIRC) and adult-onset vitelliform macular dystrophy (AVMD).