Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, RAW 264.7 cells, mouse brain tissue, C2C12 cells, 4T1 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 37 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
Product Information
66799-1-Ig targets Bcl2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24261 Product name: Recombinant mouse Bcl2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-236 aa of NM-009741 Sequence: MAQAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDADAAPLGAAPTPGIFSFQPESNPMPAVHRDMAARTSPLRPLVATAGPALSPVPPVVHLTLRRAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK 相同性解析による交差性が予測される生物種 |
| Full Name | B-cell leukemia/lymphoma 2 |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | NM-009741 |
| Gene Symbol | Bcl2 |
| Gene ID (NCBI) | 12043 |
| RRID | AB_2882142 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P10417 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.
Publications
| Species | Application | Title |
|---|---|---|
Circ Res Spatiotemporal ATF3 Expression Determines VSMC Fate in Abdominal Aortic Aneurysm | ||
Redox Biol Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload. | ||
MedComm (2020) Partial reduction of interleukin-33 signaling improves senescence and renal injury in diabetic nephropathy | ||
Phytomedicine Huangjin Shuangshen Jiawei decoction alleviated coronary heart disease comorbid with depression partly through gut microbiota-mediated regulation of the SRSF1-p53-ASPP1 signaling axis. | ||
Phytomedicine Puerarin prevents sepsis-associated encephalopathy by regulating the AKT1 pathway in microglia |