Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 26593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 26593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 26593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 26593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 26593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 26593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 26593-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 26593-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of C2C12 using 26593-1-AP
C2C12 cells were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse spleen using 26593-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 26593-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 26593-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 26593-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 26593-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of NIH/3T3 using 26593-1-AP
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Bcl2 antibody (26593-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of NIH/3T3 using 26593-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 26593-1-AP (Bcl2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of NIH/3T3 using 26593-1-AP
1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Bcl2 Polyclonal antibody (26593-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

Bcl2 Polyclonal antibody

Bcl2 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 26593-1-AP

Publications(1616)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (6)

Applications

WB, IHC, IF, FC (Intra), CoIP, ELISA

Synonyms

Bcl-2, B cell leukemia/lymphoma 2, Bcl 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1616)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	NIH/3T3 cells, C2C12 cells, mouse brain tissue, mouse heart tissue, mouse testis tissue, mouse kidney tissue, mouse spleen tissue, rat spleen tissue, rat spleen tissue., rat kidney tissue, rat testis tissue, mouse colon tissue
Positive IHC detected in	mouse spleen tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 1443 publications below
IHC	See 90 publications below
IF	See 41 publications below
CoIP	See 1 publications below

Product Information

26593-1-AP targets Bcl2 in WB, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity	mouse, rat
Cited Reactivity	mouse, rat, pig, rabbit, canine, chicken, hamster, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag24260 Product name: Recombinant mouse Bcl2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-236 aa of NM-009741 Sequence: MAQAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDADAAPLGAAPTPGIFSFQPESNPMPAVHRDMAARTSPLRPLVATAGPALSPVPPVVHLTLRRAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK 相同性解析による交差性が予測される生物種
Full Name	B-cell leukemia/lymphoma 2
Calculated molecular weight	26 kDa
Observed molecular weight	26 kDa
GenBank accession number	NM-009741
Gene Symbol	Bcl2
Gene ID (NCBI)	12043
RRID	AB_2818996
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P10417
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

1. What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2. FAQs for Bcl-2

How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.

Protocols

Product Specific Protocols
FC protocol for Bcl2 antibody 26593-1-AP	Download protocol
IF protocol for Bcl2 antibody 26593-1-AP	Download protocol
IHC protocol for Bcl2 antibody 26593-1-AP	Download protocol
WB protocol for Bcl2 antibody 26593-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Signal Transduct Target Ther Nucleosome assembly protein-like 1 degradation-dependent novel cardioprotection mechanism of Wnt2 against ischemia‒reperfusion injury Authors - Ying Wang View Article
rat	WB	Adv Mater Poly(Citric Acid) Bidirectional Regulator: Coordinating Iron Homeostasis to Suppress Stress Responses and Boosting Mitochondrial Bioenergetics for Enhanced Nerve Repair. Authors - Xinyue Liang View Article
rat,mouse	WB	Adv Mater Dual Single-Atom Engineered NADH Oxidase-Mimic Boosts In Situ NAD(+) Biotransformation for Targeted Myocardial Ischemia/Reperfusion Protection. Authors - Jingtao Liu View Article
	WB	Cancer Res Tumor-Resident Streptococcus pneumoniae Promotes Malignant Progression and Pazopanib Resistance in Clear Cell Renal Cell Carcinoma Authors - Jinpeng Wang View Article
mouse	WB	Kidney Int Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis Authors - Dong Zhou View Article
mouse	WB	Nat Commun Targeting LRBA triggers CTLA4 degradation and antitumor immunity for cancer immunotherapy. Authors - Xiaolu Ge View Article

Validation Data Gallery

WB analysis using 26593-1-AP

WB analysis using 26593-1-AP

WB analysis using 26593-1-AP

WB analysis using 26593-1-AP

WB analysis using 26593-1-AP

WB analysis using 26593-1-AP

WB analysis of mouse testis using 26593-1-AP

WB analysis of mouse heart using 26593-1-AP

WB analysis of C2C12 using 26593-1-AP

IHC staining of mouse spleen using 26593-1-AP

IHC staining of mouse brain using 26593-1-AP

IHC staining of mouse brain using 26593-1-AP

IHC staining of mouse spleen using 26593-1-AP

IHC staining of mouse spleen using 26593-1-AP

IF Staining of NIH/3T3 using 26593-1-AP

IF Staining of NIH/3T3 using 26593-1-AP

FC experiment of NIH/3T3 using 26593-1-AP

Bcl2 Polyclonal antibody

Bcl2 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Nucleosome assembly protein-like 1 degradation-dependent novel cardioprotection mechanism of Wnt2 against ischemia‒reperfusion injury

Poly(Citric Acid) Bidirectional Regulator: Coordinating Iron Homeostasis to Suppress Stress Responses and Boosting Mitochondrial Bioenergetics for Enhanced Nerve Repair.

Dual Single-Atom Engineered NADH Oxidase-Mimic Boosts In Situ NAD(+) Biotransformation for Targeted Myocardial Ischemia/Reperfusion Protection.

Tumor-Resident Streptococcus pneumoniae Promotes Malignant Progression and Pazopanib Resistance in Clear Cell Renal Cell Carcinoma

Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis

Targeting LRBA triggers CTLA4 degradation and antitumor immunity for cancer immunotherapy.

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