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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using Bche Recombinant antibody (85722-4-RR)

    WB analysis of mouse liver using 85722-4-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 85722-4-RR (Bche antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using Bche Recombinant antibody (85722-4-RR)

    WB analysis of mouse lung using 85722-4-RR

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 85722-4-RR (Bche antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using Bche Recombinant antibody (85722-4-RR)

    WB analysis of mouse heart using 85722-4-RR

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 85722-4-RR (Bche antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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BCHE Recombinant antibody

BCHE Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85722-4-RR
Clone No.250086C5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II, Cholinesterase, EC:3.1.1.8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse liver tissue, mouse heart tissue, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85722-4-RR targets BCHE in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCHE fusion protein Eg2899 相同性解析による交差性が予測される生物種
Full Name butyrylcholinesterase
Calculated molecular weight68 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberNM_009738.3
Gene Symbol Bche
Gene ID (NCBI) 12038
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03311
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Butyrylcholinesterase (BCHE) is an important cholinesterase enzyme involved in the hydrolysis of choline esters. It plays a crucial role in neurotransmitter metabolism, drug metabolism, and various physiological processes. BCHE is highly expressed in the liver, lungs, brain, and heart, but its expression levels vary in different tissues. BCHE-related research helps understand its roles in physiological and pathological processes and provides an important basis for drug development and disease diagnosis.

Protocols

Product Specific Protocols
WB protocol for BCHE antibody 85722-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols