Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 19491-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19491-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 19491-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 19491-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 19491-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 19491-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 19491-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ATG14/Barkor (N-terminal) Polyclonal antibody

ATG14/Barkor (N-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19491-1-AP

Publications(37)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Barkor, ATG14, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, Atg14L

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (37)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 35 publications below
IF	See 7 publications below
IP	See 1 publications below

Product Information

19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, canine, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	KIAA0831
Calculated molecular weight	55 kDa
Observed molecular weight	65-68 kDa
GenBank accession number	NM_014924
Gene Symbol	ATG14
Gene ID (NCBI)	22863
RRID	AB_10642701
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6ZNE5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).

Protocols

Product Specific Protocols
IF protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP	Download protocol
IHC protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP	Download protocol
WB protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy Authors - Yang Meng View Article
human	WB	Mol Cancer PMID: 32727463 Authors - Cefan Zhou View Article
human	WB	Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia Authors - Tiemei Li View Article
human	WB	Autophagy STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I. Authors - Cefan Zhou View Article
mouse	WB	Dev Cell Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility. Authors - Yuqing Lei View Article
human	WB	Autophagy Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells. Authors - Xu Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 19491-1-AP

WB analysis of mouse brain using 19491-1-AP

WB analysis of NIH/3T3 using 19491-1-AP

WB analysis of A431 using 19491-1-AP

IHC staining of mouse brain using 19491-1-AP

IHC staining of mouse brain using 19491-1-AP

IF Staining of MCF-7 using 19491-1-AP

ATG14/Barkor (N-terminal) Polyclonal antibody

ATG14/Barkor (N-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

PMID: 32727463

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.