Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, U-251 cells, RKO cells, RT-4 cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33133-1-AP targets BXDC5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37505 Product name: Recombinant human BXDC5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-349 aa of BC016051 Sequence: MAKAGDKSGSSGKKSLKRKAAAEELQEAAGAGDGATENGVQPPKAAAFPPGFSISEIKNKQRRHLMFTRWKQQQRKEKLAAKKKLKKEREALGDKAPPKPVPKTIDNQRVYDETTVDPNDEEVAYDEATDEFASYFNKQTSPKILITTSDRPHGRTVRLCEQLSTVIPNSHVYYRRGLALKKIIPQCIARDFTDLIVINEDRKTPNGLILSHLPNGPTAHFKMSSVRLRKEIKRRGKDPTEHIPEIILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIFRSEKKVGIQELGPRFTLKLRSLQKGTFDSKYGEYEWVHKPREMDTSRRKFHL 相同性解析による交差性が予測される生物種 |
| Full Name | brix domain containing 5 |
| Calculated molecular weight | 349 aa, 40 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC016051 |
| Gene Symbol | BXDC5 |
| Gene ID (NCBI) | 80135 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H9Y2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BXDC5 (Brix domain-containing protein 5), also known as ribosome biogenesis protein RPF1, is a protein containing a Brix domain and is localized in the nucleolus. The Brix domain is a region that shares homology with the yeast protein Pitx1 (ribosome biogenesis protein BRX1). BXDC5 is a key factor in ribosome biogenesis, participating in the maturation of the 60S ribosomal subunit. This protein exerts its function by binding to specific rRNA structures and acts as a downstream effector molecule of the Ran signaling pathway, involved in ribosome biogenesis. In colorectal cancer (CRC), BXDC5 influences the occurrence and development of tumors by regulating ribosome biogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BXDC5 antibody 33133-1-AP | Download protocol |
| WB protocol for BXDC5 antibody 33133-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |