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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BXDC1 Polyclonal antibody (17584-1-AP)

    WB analysis of HeLa using 17584-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17584-1-AP (BXDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BXDC1 Polyclonal antibody (17584-1-AP)

    WB analysis using 17584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17584-1-AP (BXDC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using BXDC1 Polyclonal antibody (17584-1-AP)

    WB analysis of HL-60 using 17584-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17584-1-AP (BXDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BXDC1 Polyclonal antibody (17584-1-AP)

    IF Staining of HeLa using 17584-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17584-1-AP (BXDC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BXDC1 Polyclonal antibody (17584-1-AP)

    IF Staining of HeLa using 17584-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BXDC1 antibody (17584-1-AP) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

BXDC1 Polyclonal antibody

BXDC1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17584-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

RPF2, Brix domain-containing protein 1, brix domain containing 1, bA397G5.4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HL-60 cells, HEK-293T cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17584-1-AP targets BXDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11268

Product name: Recombinant human BXDC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-297 aa of BC035314

Sequence: MDTLDRVVKPKTKRAKRFLEKREPKLNENIKNAMLIKGGNANATVTKVLKDVYALKKPYGVLYKKKNITRPFEDQTSLEFFSKKSDCSLFMFGSHNKKRPNNLVIGRMYDYHVLDMIELGIENFVSLKDIKNSKCPEGTKPMLIFAGDDFDVTEDYRRLKSLLIDFFRGPTVSNIRLAGLEYVLHFTALNGKIYFRSYKLLLKKSGCRTPRIELEEMGPSLDLVLRRTHLASDDLYKLSMKMPKALKPKKKKNISHDTFGTTYGRIHMQKQDLSKLQTRKMKGLKKRPAERITEDHE

相同性解析による交差性が予測される生物種
Full Name brix domain containing 1
Calculated molecular weight 306 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC035314
Gene Symbol BXDC1
Gene ID (NCBI) 84154
RRIDAB_1939419
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H7B2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BXDC1 antibody 17584-1-APDownload protocol
IF protocol for BXDC1 antibody 17584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols