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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BUD13 Polyclonal antibody

BUD13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20163-1-AP

Synonyms

BUD13, BUD13 homolog, BUD13 homolog (S. cerevisiae), fSAP71

Validation Data Gallery

Applications:
  • WB analysis using 20163-1-AP

    WB analysis using 20163-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20163-1-AP (BUD13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 20163-1-AP

    IHC staining of mouse testis using 20163-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20163-1-AP (BUD13 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 20163-1-AP

    IHC staining of mouse testis using 20163-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20163-1-AP (BUD13 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, PC-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

20163-1-AP targets BUD13 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BUD13 fusion protein Ag13665 相同性解析による交差性が予測される生物種
Full Name BUD13 homolog (S. cerevisiae)
Calculated molecular weight 619 aa, 71 kDa
Observed molecular weight 71-85 kDa
GenBank accession numberBC006350
Gene symbol BUD13
Gene ID (NCBI) 84811
RRIDAB_2878650
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BUD13 homolog (BUD13) is a component of the retention and splicing (RES) complex which was previously identified in yeast as a splicing factor affecting nuclear pre-mRNA retention. As an RNA binding protein (RBP), BUD13 expression is associated with poor prognosis and may be involved in the development and progression of hepatocellular carcinoma (PMID: 37605593).

Protocols

Product Specific Protocols
WB protocol for BUD13 antibody 20163-1-APDownload protocol
IHC protocol for BUD13 antibody 20163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols