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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BUB3 Recombinant antibody (83266-5-RR)

    WB analysis using 83266-5-RR (same clone as 83266-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83266-5-RR (BUB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BUB3 Recombinant antibody (83266-5-RR)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BUB3 Recombinant antibody (83266-5-RR)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BUB3 Recombinant antibody (83266-5-RR)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using BUB3 Recombinant antibody (83266-5-RR)

    FC experiment of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BUB3 Recombinant antibody (83266-5-RR, Clone:240087D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83266-5-RR

    Affinity and Kinetic Characterization of 83266-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83266-5-RR against Human BUB3 were performed. The affinity constant is 0.228 nM.

BUB3 Recombinant monoclonal antibody, PBS Only

BUB3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83266-5-PBS
Clone No.240087D9

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

hBUB3, BUB3L, BUB 3, 240087D9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83266-5-PBS targets BUB3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25608

Product name: Recombinant human BUB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 205-328 aa of BC005138

Sequence: VEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT

相同性解析による交差性が予測される生物種
Full Name budding uninhibited by benzimidazoles 3 homolog (yeast)
Calculated molecular weight 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC005138
Gene Symbol BUB3
Gene ID (NCBI) 9184
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO43684
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.