Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24959-1-AP targets BTNL8 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21203 Product name: Recombinant human BTNL8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 90-147 aa of BC119696 Sequence: CSMRHAHLSREVESRVQIGDTFFKPISWHLATKVLGILCCGLFFGIVGLKIFFSKFQW 相同性解析による交差性が予測される生物種 |
| Full Name | butyrophilin-like 8 |
| Calculated molecular weight | 500 aa, 57 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC119696 |
| Gene Symbol | BTNL8 |
| Gene ID (NCBI) | 79908 |
| RRID | AB_2879822 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UX41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BTNL8 (butyrophilin-like 8) belongs to the butyrophilin (BTN) family, a group of immunoglobulin superfamily members structurally and functionally related to the B7 family of immune costimulatory molecules. The protein is predicted to localize to the plasma membrane and is involved in T cell receptor signaling and regulation of cytokine production. It is highly expressed in the gastrointestinal tract, particularly in the duodenum and small intestine, as well as in the colon and rectum.BTNL8 is also expressed in neutrophils. Functionally, BTNL8 plays a role in modulating immune responses. It can augment antigen-induced activation of T lymphocytes, promoting T-cell proliferation and cytokine production.(PMID:24036152)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BTNL8 antibody 24959-1-AP | Download protocol |
| WB protocol for BTNL8 antibody 24959-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |