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Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using BTN3A2 Polyclonal antibody (15148-1-AP)

    WB analysis of SKOV-3 using 15148-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15148-1-AP (BTN3A2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using BTN3A2 Polyclonal antibody (15148-1-AP)

    WB analysis of SKOV-3 using 15148-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15148-1-AP (BTN3A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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BTN3A2 Polyclonal antibody

BTN3A2 Polyclonal Antibody for WB, ELISA
Cat No. 15148-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

BT3.2, BT3.3, BTF3, BTF4, BTN3A2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15148-1-AP targets BTN3A2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTN3A2 fusion protein Ag2212 相同性解析による交差性が予測される生物種
Full Name butyrophilin, subfamily 3, member A2
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC020214
Gene Symbol BTN3A2
Gene ID (NCBI) 11118
RRIDAB_2878112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78410
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTN3A2, also named as Butyrophilin subfamily 3 member A2, is a 334 amino acid protein, which contains 1 Ig-like V-type (immunoglobulin-like) domain and belongs to the immunoglobulin superfamily. BTN3A2 plays a role in T-cell responses in the adaptive immune response and inhibits the release of IFNG from activated T-cells. BTN3A2 can form Homodimer and is detected in T-cells and natural killer cells.

Protocols

Product Specific Protocols
WB protocol for BTN3A2 antibody 15148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols