Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84613-2-RR targets BTD in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | BTD fusion protein Ag9449 相同性解析による交差性が予測される生物種 |
| Full Name | biotinidase |
| Calculated molecular weight | 543 aa, 61 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | BC012099 |
| Gene Symbol | BTD |
| Gene ID (NCBI) | 686 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43251 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BTD(Biotinidase) functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in the regeneration of free biotin. BTD has also been shown to have biotinyl transferase activity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BTD antibody 84613-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |