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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis using 66683-1-Ig (same clone as 66683-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis of NCCIT using 66683-1-Ig (same clone as 66683-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis of A549 using 66683-1-Ig (same clone as 66683-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-231 cells using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis of MDA-MB-231 using 66683-1-Ig (same clone as 66683-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis of T-47D using 66683-1-Ig (same clone as 66683-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis of PC-3 using 66683-1-Ig (same clone as 66683-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

Betacellulin Monoclonal antibody, PBS Only

Betacellulin Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66683-1-PBS
Clone No.2H5E8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

BTC, Probetacellulin, 2H5E8

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66683-1-PBS targets Betacellulin in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26724

Product name: Recombinant human BTC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 32-118 aa of BC011618

Sequence: DGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVCDEGYIGARCERVDLFYLRGDRGQ

相同性解析による交差性が予測される生物種
Full Name betacellulin
Calculated molecular weight 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC011618
Gene Symbol BTC
Gene ID (NCBI) 685
RRIDAB_2882037
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP35070
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, binds and activates ErbB1 and ErbB4 homodimers. BTC is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. BTC was expressed in tumors and involved in tumor growth progression.

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