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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using CD157 Polyclonal antibody (16337-1-AP)

    WB analysis of RAW 264.7 using 16337-1-AP

    WB result of CD157 antibody (16337-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD157 transfected RAW 264.7 cells.

  • Western Blot (WB) analysis of A549 cells using CD157 Polyclonal antibody (16337-1-AP)

    WB analysis of A549 using 16337-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16337-1-AP (CD157 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD157 Polyclonal antibody (16337-1-AP)

    IHC staining of human tonsillitis using 16337-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16337-1-AP (CD157 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD157 Polyclonal antibody (16337-1-AP)

    IHC staining of human tonsillitis using 16337-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16337-1-AP (CD157 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO Validated

CD157 Polyclonal antibody

CD157 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16337-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

ADP ribosyl cyclase 2, ADP-ribosyl cyclase 2, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, Bone marrow stromal antigen 1, BST 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16337-1-AP targets CD157 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD157 fusion protein Ag9491 相同性解析による交差性が予測される生物種
Full Name bone marrow stromal cell antigen 1
Calculated molecular weight 318 aa, 36 kDa
Observed molecular weight 39-46 kDa
GenBank accession numberBC012095
Gene Symbol CD157
Gene ID (NCBI) 683
RRIDAB_2878244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10588
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BST-1(bone marrow stromal cell antigen 1), also named as CD157 or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. BST-1 has a calculated molecular mass of 30-36 kDa and an apparent molecular mass of 40-46 kDa due to the glycosylation (PMID: 8941363, 10491089).

Protocols

Product Specific Protocols
WB protocol for CD157 antibody 16337-1-APDownload protocol
IHC protocol for CD157 antibody 16337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

EMBO J

Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function

Authors - Quanjiang Zhang
View Article