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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRWD1 Polyclonal antibody (29293-1-AP)

    WB analysis using 29293-1-AP (same clone as 29293-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BRWD1 Polyclonal antibody (29293-1-AP)

    WB analysis using 29293-1-AP (same clone as 29293-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BRWD1 Polyclonal antibody (29293-1-AP)

    IHC staining of mouse cerebellum using 29293-1-AP (same clone as 29293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BRWD1 Polyclonal antibody (29293-1-AP)

    IHC staining of mouse cerebellum using 29293-1-AP (same clone as 29293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

BRWD1 Polyclonal antibody, PBS Only

BRWD1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29293-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Bromodomain and WD repeat-containing protein 1, C21orf107, N143, WD repeat containing protein 9, WD repeat-containing protein 9

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29293-1-PBS targets BRWD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30902

Product name: Recombinant human BRWD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-120 aa of BC064602

Sequence: MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKGTLI

相同性解析による交差性が予測される生物種
Full Name bromodomain and WD repeat domain containing 1
Calculated molecular weight 2320 aa, 263 kDa
Observed molecular weight263 kDa
GenBank accession numberBC064602
Gene Symbol BRWD1
Gene ID (NCBI) 54014
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSI6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BRWD1 (Bromodomain and WD repeat domain containing 1, also known as WDR9) has a predicted molecular weight of 263 kDa and contains tandem BROMO domains and WD40 repeats (PMID:12889071). One of the remarkable functions of BRWD1 was the coordinated repression of MYC and MYC target genes. At the Myc/MYC locus, in both mice and humans, BRWD1 specififically repressed distal enhancers, which are associated with lineage specifific expression (PMID:30250168). In addition,BRWD1 as a key epigenomic mediator of normal neurodevelopment and an important contributor to DS (Down syndrome)-related phenotypes (PMID:36289231).

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